I ran benchmarks on my 09 MP 2.26 octo before and after adding the new memory.
The test were run using diglloydTools Stress Test v 1.1.0 64 bit.
With 12 gb, 6 x 2 gb the max memory speed was 10,421 MB/sec.
With 16 gb, 8 x 2 gb the max memory speed was 9,024 MB/sec.
There is a slight performance penalty, but as cmaier has pointed out, the penalty is greatly offset if you have to page to hard disk. Also, OS X does a good job of caching applications in your memory.
You mileage may vary! 🙂