I remuxed about 30 mkv files into m4v to put into iTunes and use with an Apple TV 3 and I'm cleaning them up with Subler. I'm using the program to put the stereo track before the AC3 track and make sure all the tracks are labeled properly. Then I optimize the file so it will stream more efficiently. My question is, if I decide to go into the file after I finish optimizing it and add metadata or adjust something, do I need to optimize it again after saving it? Thanks!