When testing the RAM stick one by one, you may blow all the dust away from the slot as well.
In my own experence. There is no need to do PRAM reset after RAM config change. However, quite a few members here report that a PRAM reset fix their RAM issue (especially wrong RAM speed). So, unless you are running a unflashed Maxwell GPU. Or still using the old method in Yosemite to activate TRIM. Reset PRAM should not cause any damage / trouble. Always worth a try before other trouble shooting.