Are you saying first build a raid 0 (2-4 disks) then partition that array into 2 partitions so that the OS sees 2 independent disks?
Other way around - partition the stripes, then make separate arrays. Sure, creating a striped array on disks used for nothing else provides a greater boost than doing multiple things with them, but depending on their use, you can still see a major speed up. A huge chunk of my disks are used for backups - backups run when I'm not using the computer, so there's no competition for disk I/O.
My G5 was had a scratch array striped across multiple disks, using only a small partition from each. It provided a boost in Photoshop performance.
When my new WD Black drives arrive for my new Mac Pro, I'll be taking 15GB off the front of each disk for a striped array. The rest of the space on each disk (a - d) will be used for a) Boot/Applications + Backup of drives b and c; b) User Data, Backup of drive a; c) Games, Media, Windows crap; d) Time Machine
Would there even be a performance gain from doing so?
Depends on your usage. If a given disk is being intensively accessed for multiple things at once, then the gain won't be large. If your Photoshop scratch disk is being hammered, what else is your computer doing with those disks? (The answer may or may not be 'nothing' for you. It's typically 'not much' for me)
If your usage is 'launch app' -> 'load files from disk' -> 'do intensive things' -> 'save', then what should you optimise?
You're reading the application
onceRAID1 makes more sense for the boot volume than RAID0, if you've split your data off it - but really, how long does it take a Mac Pro to launch your app? after the first time it's probably in RAM anyway. For me, my Mac is on 24/7 and I have enough RAM that there's a good chance my app is either running or cached.
You probably want to optimise the other steps. If you're using Photoshop, you aren't loading/saving while doing anything else, so your optimisations can be at odds.
Read this:
http://macperformanceguide.com/OptimizingPhotoshop-Configuration.html
Like PowerPaw, I use partitions for ease of migration and backup. Performance was secondary. My G5's partitions aren't in the right spots for the best performance, the Mac Pro's will be much better planned (partitions at the start of the disk are faster than those at the back).