There could be a large degree of things going on, but in the very essence of computer engineering, you can expect a computer to consume space as it runs without your explicit interaction relating to that. Instances such as Safari caches, Adobe caching things, temporary files, Local Time Machine backups, memory paging, HFS rearrangement of files on disk, and endless more can cause minor shifts in the used space on a hard drive.
There are other situations that are worrisome, like a hard drive failure, though they often come with other side effects. Does this number continuously rise to where it's a common trend? When you restart does the number seem to be increasing?
Also in the Activity Monitor, under the Disk tab, you can see what processes are reading/writing the disk which may help you pinpoint some sort of rogue execution.