I went with 2.26 with 160gb.
I already upgraded memory to 4GB (about $50 with rebate from newegg)
IMHO, the clock speed difference between 2.53 and 2.26 doesn't justify $200. And with $200, I think getting SSD HDD will dramatically improve the speed of operations with MBP.
Also if you do web design, I guess you will be using photoshop and deal with many digital photos so having SSD will definitely be a big plus.
Some argue that 2.53-2.26 = 0.27 is not the case since the CPU is Core2Duo which makes it 0.27 x 2 = 0.54 Ghz difference. (Just something to ponder.

)
Well SSD is also very expensive at the moment but Intel is releaseing new line of SSD so price will fall in due time.
There are many SSD articles floating around the web so make sure that is something you would like to do.
I am not sure about Apple store installing 3rd party upgrades. (best to go and visit genius at the

store) Installing memory wasn't all that hard though.
I do all the heavy lifting on my desktop so I can't say for 100% that 2.56 is not worth $200 but using the MBP for surfing/movies/MP3s/coding with 2.26 is more than enough IMHO.
Well those were my 2cents,
Im going to purchase a new 13" macbook pro and am debating wether or not I need to get the 2.26ghz or 2.53ghz. If I got the 2.26 I would upgrade the ram to 4gb and the hard drive to 250gb. So is it worth the 200$ to get the 2.53 processor? I would just be using the computer for web surfing, some games, and mostly website design.
Thanks,
P.S.
Can the apple store customize the macbook pro at the store? Or would I have to order it online? (I don't feel comfortable changing the hard drive and ram myself)