Note that suppressing the creation of .DS_Store files is only possible on network drives such as file servers – they will still always be created on local drives, including flash drives or anything that appears as a flash drive. The creation of .DS_Store and related files on flash-like drives is known to cause problems with some embedded devices that do not expect those files, including several popular models of digital photo frames.
Not really, but you can keep it from being modified. go into terminal use rm to get rid of the existing .ds_store and then use touch .ds_store to create a new blank one. then lock the file 🙂