Yes, you can load 3GB of DDR3 RAM into a unibody macbook, you just won't have the "benefit" of dual-channel RAM since they aren't a matched pair; but many benchmarks suggest that the effect of more RAM is greater than the effect of dual-channel with less RAM.
I'd wager you'll never notice the loss of dual channel day-to-day, but will see the increased RAM as a performance boost.