Yes and no. Technically it can work, but you have to do a lot of modifications. You'd need to purchase a slimline IDE to desktop IDE adapter, as well as a converter for laptop to desktop power cable. I believe you'd also need some new mounting brackets as the original optical drive in the iMac is far larger than the new type laptop drives. I did the same modification in my Cube (which uses the same drive as the iMac), so i know it does work, but takes some effort.