Sooo.. since I was curious...
I used the iDefrag Demo to "analyze" one of my drives, and then I used SuperDuper! to clone that drive to another drive, and then used iDefrag Demo to "analyze" that cloned drive... Here are the results.
Fragmented disk "Dharma":
http://img526.imageshack.us/img526/2953/frag1nm9.th.jpg
http://img339.imageshack.us/img339/7834/frag2gu7.th.jpg
Cloned Disk "Losties":
http://img339.imageshack.us/img339/1820/2frag1rx3.th.jpg
http://img522.imageshack.us/img522/2019/2frag2dh4.th.jpg
Make of this what you will.
I will note this, however:
While I will agree that a defragmentation makes a significant impact upon a drive that is incredibly fragmented (we're talking 60% or more), much of the time, people that defrag as a maintenance task only PERCEIVE their drive to be "faster". We're talking between a second and tenths of seconds. We see the same attitude with people who repair permissions and run maintenance scripts who claim their drives run faster. They don't. They just perceive them to because they've done "maintenance".