K, get ready to freak out. I had this problem very intermittantly - it didn't bother me as it would only last for a few seconds - until this morning. Now the backlight will not turn on for more than one second at a time. Updated to 3.0.1, restored, reset all settings, etc. and all that happens is the back light stays on for about 1-2 seconds after waking from standby and then turns off.
Halp!
Just take it to an Apple store...why freak out?
I know MR is a great place to find troubleshooting advice, but if you have a 3GS, you're still under warranty and you still can get technical service over the phone for free....
You'd think more people would take advantage of this...